Physical and Chemical Characteristics of Chloroplast Fragments. Applied Research Concerning Artificial Photosynthesis

Item

Title
Physical and Chemical Characteristics of Chloroplast Fragments. Applied Research Concerning Artificial Photosynthesis
Date
1962
Index Abstract
Not Available
Photo Quality
Complete
Report Number
AMRL TDR 62-146
Creator
Gross, Jean A.
Becker, Milton J.
Shefner, Alan M.
Corporate Author
Armour Research Foundation
Laboratory
Biomedical Laboratory
Extent
51
PB Number
PB113340
Identifier
AD0296951
AD0296951
Access Rights
OTS
Distribution Classification
1
Contract
AF 33(616)-7255
DoD Project
7164
DoD Task
716403
DTIC Record Exists
Yes
Distribution Change Authority Correspondence
None
Distribution Conflict
No
Cover Price
$1.50
Abstract
Chloroplasts isolated from spinach leaves were physically fragmented and then fractionated by differential centrifugation. A high-activity fraction, designated CF(20-50), was obtained and retained its high activity regardless of environmental changes. It had a low protein content and a relatively high chlorophyll-to-protein ratio. Plastoquinone was present in high concentration. Extraction of plastoquinone from CF(20-50) inhibited Hill activity. Unlike whole chloroplasts, readdition of plastoquinone failed to induce even partial recovery. The average particle size in CF(20-50) was 1500 angstroms in diameter. The average sedimentation constant was 800; the molecular weight was about 8 x 10 to the 7th power; and it showed three electrophoretic bands. CF(20-50) showed maximal Hill activity, but was not the smallest photo-active unit studied. Although the nature of the smallest functional unit is uncertain, it is clear that a CF(20-50) particle is composed of smaller sub-units probably oriented to impart high activity to the larger composite structure.
Report Availability
Full text available
Corporate Report Number
ARF 3174-24
Date Issued
1962-12
Index In
U.S. Government Research Reports, Vol. 38, No. 10, p. 53
Index Price
$1.50
Provenance
RAF Centre of Aviation Medicine
Type
report
Format
1 online resource
Item sets
Illinois Tech Related
Media
AMRLTDR62-146.pdf