Title

Effects of Cold Stress on Cellular Structure and Function

Date

1966

Index Abstract

Not Available

Photo Quality

Complete

Report Number

AMRL TR 66-30

Creator

Buchsbaum, Ralph

Buchsbaum, Monte

Linsenmayer, Thomas

Corporate Author

University of Pittsburgh

Laboratory

Aerospace Medical Research Laboratories

Extent

15

Identifier

AD0638327

Access Rights

Distribution of this document is unlimited.

Distribution Classification

1

Contract

AF 33(615)-1076

DoD Project

7164

DoD Task

716409

DTIC Record Exists

No

Distribution Change Authority Correspondence

None

Abstract

The purpose of this investigation was to ascertain more precisely the limits of tolerance of cells in vitro to cold, as a basis for investigations on the cellular mechanisms affected. Mouse kidney cells were maintained in a perfusion chamber arranged for cooling and warming at various rates. Normal tissue culture media were used. Nearly all cells exposed to -0.3 C for 10 minutes, then warmed to 37 C, lived. Nearly all cells exposed to -1 C or lower for 10 minutes, and warmed to 37 C, died. The critical lethal cold shock seems to be between -0.3 and -0.7 C. Cold shock seems to produce little obvious change in cells, but mitochondrial breaks and swelling are obivous in electron micrographs. Also, nuclear ground substance appeared more granular than normal. One hypothesis was that microcrystallization probably occurs in certain organelles, particularly the mitochondria because these organelles are in constant motion. Similar results were obtained in experiments with human neutrophiles.

Report Availability

Full text available

Date Issued

1966-05

Provenance

RAF Centre of Aviation Medicine

Type

report

Format

1 online resource